Tuberin antibody (N-Term)

Catalog No. ABIN501075

This anti-Tuberin antibody is a Rabbit Polyclonal antibody detecting Tuberin in WB, IF and EIA. Suitable for Human and Mouse.

Quick Overview for Tuberin antibody (N-Term) (ABIN501075)

Target: Tuberin (TSC2) (Tuberous Sclerosis 2 (TSC2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tuberin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-Tuberin Antibody

Binding Specificity: N-Term

Specificity: This antibody detects TSC2 at N-term.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse

Purification: Peptide affinity chromatography

Immunogen: TSC2 (NT) was raised against a 14 amino acid peptide from near the amino terminus of human TSC2.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 μg/mL. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for Tuberin

Target: Tuberin (TSC2) (Tuberous Sclerosis 2 (TSC2))

Alternative Name: Tuberin / TSC2

Background: Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1,2). TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high (3). Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro (4), suggesting that the TSC1/TSC2 complex decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing that overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth (4,5). At least three isoforms of TSC2 exist.Synonyms: TSC4, Tuberous sclerosis 2 protein

Gene ID: 7249

NCBI Accession: NP_000539

Pathways: RTK Signaling, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus