UNG antibody (N-Term)

Catalog No. ABIN501108

This anti-UNG antibody is a Rabbit Polyclonal antibody detecting UNG in WB, IHC (p) and EIA. Suitable for Human, Mouse and Rat.

Quick Overview for UNG antibody (N-Term) (ABIN501108)

Target: UNG (Uracil-DNA Glycosylase (UNG))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UNG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-UNG Antibody

Binding Specificity: N-Term

Specificity: This antibody detects Uracil-DNA glycosylase (UNG) at N-term. The antibody will not cross-react with UNG2.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Affinity chromatography

Immunogen: UNG1 antibody was raised against a 13 amino acid peptide from near the amino terminus of human UNG1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for UNG

Target: UNG (Uracil-DNA Glycosylase (UNG))

Background: The human uracil-DNA glycosylase (UNG) gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms through differentially regulated promotes and alternative splicing. While UNG2 is the major enzyme in the base excision repair pathway that removes uracil residues from nuclear DNA that arise through either misincorporation during replication or cytosine deamination, inhibition of UNG1 by uracil glycosylase inhibitor did not lead to increased levels of spontaneous or induced mitochondrial DNA mutations. However, decreased levels of UNG activity and increased oxidative damage to mitochondrial DNA were seen in older mice, suggesting that mitochondrial DNA repair mechanisms may be involved in various neurodegenerative disorders in an age-dependent manner.Synonyms: DGU, UDG, UNG1, UNG15

Gene ID: 7374

NCBI Accession: NP_003353

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response