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Vimentin antibody (AA 101-200)

Name: Vimentin antibody (AA 101-200)
SKU: ABIN5012405
Availability: InStock

The Rabbit Polyclonal anti-Vimentin antibody (ABIN5012405) specifically detects Vimentin in WB, ELISA, FACS, IHC (p), ICC, IF (p), IHC (fro) and IF (cc). The antibody is reactive with Human samples.

Lane 1: U2OS Cell lysates; Lane 2: U87MG Cell lysates; Lane 3: HeLa lysates; Lane 4: Jurkat lysates; probed with Vimentin Polyclonal Antibody, unconjugated (bs-23063R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C. (Vimentin antibody (AA 101-200))

Paraformaldehyde-fixed, paraffin embedded human cervical cancer section; Antigen retrieval by boiling in sodium citrate buffer (pH6. (Vimentin antibody (AA 101-200))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded human liver carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6. (Vimentin antibody (AA 101-200))

IHC (p)

3 images

Catalog No. ABIN5012405

$384.62

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 4 to 7 Business Days

Quick Overview for Vimentin antibody (AA 101-200) (ABIN5012405)

Target

See all Vimentin (VIM) Antibodies

Vimentin (VIM)

Reactivity

Human

Host

Rabbit

Clonality

Polyclonal

Conjugate

This Vimentin antibody is un-conjugated

Application

Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc))

Discover our top product VIM Primary Antibody

Binding Specificity
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AA 101-200

Cross-Reactivity

Human

Predicted Reactivity

Mouse,Rat,Cow,Sheep,Horse

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human Vimentin

Isotype

IgG
anti-Vimentin (VIM) (AA 371-466) antibody
VIM Reactivity: Human, Rat, Mouse, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IF (p), IHC (fro), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM Reactivity: Human, Rat, Mouse WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Rat, Mouse, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, IF Host: Mouse Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) (AA 41-140) antibody
VIM Reactivity: Human WB, ELISA, IF, IP Host: Mouse Monoclonal 3E9 unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

Vimentin (VIM)

Alternative Name

Vimentin

Background

Synonyms: Vimentin, VIM,
Background: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Gene ID

7431

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis