LIG1 antibody (AA 484-663)

Catalog No. ABIN5013206

Product Details anti-LIG1 Antibody

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Binding Specificity: AA 484-663

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to ATP Dependent DNA ligase I (LIG1)

Specificity: The antibody is a rabbit polyclonal antibody raised against LIG1. It has been selected for its ability to recognize LIG1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPB269Hu01Recombinant ATP Dependent DNA ligase I (LIG1)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Alternative Name: LIG1 (LIG1 Products)

Background: Polydeoxyribonucleotide synthase [ATP] 1

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA