PDK3 antibody (AA 138-396)

Catalog No. ABIN5014242

The Rabbit Polyclonal anti-PDK3 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect PDK3 in samples from Mouse.

Quick Overview for PDK3 antibody (AA 138-396) (ABIN5014242)

Target: PDK3 (Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-PDK3 Antibody

Binding Specificity: AA 138-396

Purpose: Polyclonal Antibody to Pyruvate Dehydrogenase Kinase Isozyme 3 (PDK3)

Specificity: The antibody is a rabbit polyclonal antibody raised against PDK3. It has been selected for its ability to recognize PDK3 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PDK3 (Gly138-Pro396)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for PDK3

Target: PDK3 (Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3))

Alternative Name: PDK3

Background: PDHK3, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial

Pathways: PI3K-Akt Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect