DNMT1 antibody (AA 128-429)

Catalog No. ABIN5027162

This anti-DNMT1 antibody is a Mouse Monoclonal antibody detecting DNMT1 in WB, IHC and FACS. Suitable for Human.

Quick Overview for DNMT1 antibody (AA 128-429) (ABIN5027162)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNMT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: ABM13B2

Product Details anti-DNMT1 Antibody

Binding Specificity: AA 128-429

Cross-Reactivity: Mouse (Murine)

Purification: Protein G Chromatography

Immunogen: A partial length recombinant DNMT1 protein (amino acids 128-429) was used as the immunogen for this antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: WB: 2-4 μg/mL, IHC: 5 μg/mL, FACS: 0.5 μg/10^6 cells

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Target Details for DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: Dnmt1

Background: Dnmt1(DNA (cytosine-5-)-methyltransferase 1 ) is one of the most abundant DNA methyltransferase in mammalian tissues, where it associates with the replication machinery and restores symmetrical methylation at hemimethylated CpG sites generated by the semi-conservative DNA replication process. Dnmt1 comprises a regulatory N-terminal region and a C-terminal catalytic domain connected by a linker of seven glycine-lysine repeats. The N-terminal part contains a PCNA binding domain (PBD), a heterochromatin targeting sequence (TS), a CXXC-type zinc finger domain and two Bromo-Adjacent Homology domains (BAH1 and BAH2). The C-terminal domains of mammalian Dnmts contain all ten catalytic motifs identified in bacterial DNA (cytosine-5) methyltransferases. Dnmt1 maintains methylation patterns with high fidelity and is essential for embryonic development and genome integrity. The molecules interacting with Dnmt1, including RNA polymerase II, some RNA-binding proteins, and some specific Dnmt1-inhibitory RNA molecules are involved in chromatin organization, DNA repair, cell cycle regulation, and apoptosis.

Molecular Weight: 183

Gene ID: 1786

UniProt: P26358

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection