DNMT3A antibody

Catalog No. ABIN5027477

The Mouse Monoclonal anti-DNMT3A antibody (Clone ABM13G4) (ABIN5027477) specifically detects DNMT3A in WB and FACS. The antibody is reactive with Human samples.

Quick Overview for DNMT3A antibody (ABIN5027477)

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNMT3A antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: ABM13G4

Product Details anti-DNMT3A Antibody

Purification: Protein G Chromatography

Immunogen: A partial length recombinant protein from DNMT3a was used as the immunogen for this antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: WB: 2-4 μg/mL, FACS: 0.5-1 μg/10^6

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Target Details for DNMT3A

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Alternative Name: DNMT3a

Background: Dnmt3a belongs to the mammalian methyltransferase gene family which is responsible for tissue-specific gene expression. Dnmt3a together with other methyltransferases conducts de novo methylation of cytosine residues in CpG islands by the enzymatic addition of methyl residues from S-adenosyl-L-methionine to the 5-carbon atom of the cytosine ring. Dnmt3a also directs a metabolic program by repressing key genes to enable the coupling of insulin secretion to glucose levels during beta cell maturation. Loss of Dnmt3a in pancreatic beta cells prevents this developmental metabolic reprogramming, resulting in loss of GSIS (Glucose-Stimulated Insulin Secretion)). Dnmt3a overexpression has been found in several cancers including AML (Acute Myeloid Leukemia).

Molecular Weight: 15.99

Gene ID: 1788

UniProt: Q9Y6K1