SCNN1B antibody (AA 617-638) (Biotin)

Catalog No. ABIN5066886

This Mouse Monoclonal antibody specifically detects SCNN1B in WB and IHC. It exhibits reactivity toward Rat.

Quick Overview for SCNN1B antibody (AA 617-638) (Biotin) (ABIN5066886)

Target: SCNN1B (Sodium Channel, Nonvoltage-Gated 1, beta (SCNN1B))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SCNN1B antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 7B8

Product Details anti-SCNN1B Antibody (Biotin)

Binding Specificity: AA 617-638

Specificity: Detects ~87 kDa.

Cross-Reactivity: Mouse

Purification: Protein G Purified

Immunogen: Synthetic peptide from the C-terminal of Rat ENaC beta (aa. 617-638)

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:150)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5066886 was sufficient for detection of ENaC beta in 15 μg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for SCNN1B

Target: SCNN1B (Sodium Channel, Nonvoltage-Gated 1, beta (SCNN1B))

Alternative Name: ENaC beta

Background: The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta, and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).

Gene ID: 24767

NCBI Accession: NP_036780

UniProt: P37090