SPHK1 antibody (AA 1-384)

Catalog No. ABIN522355

This Mouse Monoclonal antibody specifically detects SPHK1 in WB and ELISA. It exhibits reactivity toward Human and has been mentioned in 11+ publications.

Quick Overview for SPHK1 antibody (AA 1-384) (ABIN522355)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPHK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 1D6

Product Details anti-SPHK1 Antibody

Binding Specificity: AA 1-384

Purpose: Mouse monoclonal antibody raised against a full length recombinant SPHK1.

Sequence: MDPAGGPRGV LPRPCRVLVL LNPRGGKGKA LQLFRSHVQP LLAEAEISFT LMLTERRNHA RELVRSEELG RWDALVVMSG DGLMHEVVNG LMERPDWETA IQKPLCSLPA GSGNALAASL NHYAGYEQVT NEDLLTNCTL LLCRRLLSPM NLLSLHTASG LRLFSVLSLA WGFIADVDLE SEKYRRLGEM RFTLGTFLLL AALRTYRGRL AYLPVGRVGS KTPASPVVVQ QGPVDAHLVP LEEPVPSHWT VVPDEDFVLV LALLHSHLGS EMFAAPMGRC AAGVMHLFYV RAGVSRAMLL RLFLAMEKGR HMEYECPYLV YVPVVAFRLE PKDGKGVFAV DGELMVSEAV QGQVHPNYFW MVSGCVEPPP SWKPQQMPPP EEPL

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: SPHK1 (AAH08040, 1 a.a. ~ 384 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-SPHK1 antibody (ABIN522355)

Sheu, Lee, Hua, Li, Lai, Lee, Cheng, Chen, Chan, Chao, Chen, Chang, Lee: "LRIG1 modulates aggressiveness of head and neck cancers by regulating EGFR-MAPK-SPHK1 signaling and extracellular matrix remodeling." in: Oncogene, Vol. 33, Issue 11, pp. 1375-84, (2014) (PubMed).

Böhm, Flößer, Ermler, Fender, Lüth, Kleuser, Schrör, Rauch: "Factor-Xa-induced mitogenesis and migration require sphingosine kinase activity and S1P formation in human vascular smooth muscle cells." in: Cardiovascular research, Vol. 99, Issue 3, pp. 505-13, (2013) (PubMed).

Zhang, Wang, Zhao, Di: "MiR-124 inhibits the migration and invasion of ovarian cancer cells by targeting SphK1." in: Journal of ovarian research, Vol. 6, Issue 1, pp. 84, (2013) (PubMed).

Schenten, Melchior, Steinckwich, Tschirhart, Bréchard: "Sphingosine kinases regulate NOX2 activity via p38 MAPK-dependent translocation of S100A8/A9." in: Journal of leukocyte biology, Vol. 89, Issue 4, pp. 587-96, (2011) (PubMed).

Ali-Rahmani, Hengst, Connor, Schengrund: "Effect of HFE variants on sphingolipid expression by SH-SY5Y human neuroblastoma cells." in: Neurochemical research, Vol. 36, Issue 9, pp. 1687-96, (2011) (PubMed).

Asher, Warren, Shaw, Sowter, Bali, Khan: "The role of Eag and HERG channels in cell proliferation and apoptotic cell death in SK-OV-3 ovarian cancer cell line." in: Cancer cell international, Vol. 11, pp. 6, (2011) (PubMed).

Schröder, Richter, Juan, Maltusch, Giegold, Quintini, Pfeilschifter, Huwiler, Radeke: "The sphingosine kinase 1 and S1P1 axis specifically counteracts LPS-induced IL-12p70 production in immune cells of the spleen." in: Molecular immunology, Vol. 48, Issue 9-10, pp. 1139-48, (2011) (PubMed).

Sinha, Schorn, Hochstim, Chinn, Zhu, Masood: "Increased radiation sensitivity of head and neck squamous cell carcinoma with sphingosine kinase 1 inhibition." in: Head & neck, Vol. 33, Issue 2, pp. 178-88, (2011) (PubMed).

Wang, Zhang, Lin, Xu, Feng, Ding: "A polysaccharide, MDG-1, induces S1P1 and bFGF expression and augments survival and angiogenesis in the ischemic heart." in: Glycobiology, Vol. 20, Issue 4, pp. 473-84, (2010) (PubMed).

Billich, Urtz, Reuschel, Baumruker: "Sphingosine kinase 1 is essential for proteinase-activated receptor-1 signalling in epithelial and endothelial cells." in: The international journal of biochemistry & cell biology, Vol. 41, Issue 7, pp. 1547-55, (2009) (PubMed).

in: (PubMed).

Target Details for SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Alternative Name: SPHK1

Background: Full Gene Name: sphingosine kinase 1
Synonyms: SPHK

Gene ID: 8877

Pathways: VEGF Signaling