CBFB antibody (N-Term)

Catalog No. ABIN5514455

The Rabbit Polyclonal anti-CBFB antibody (ABIN5514455) specifically detects CBFB in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Horse, Dog, Rabbit and Guinea Pig samples.

Quick Overview for CBFB antibody (N-Term) (ABIN5514455)

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Reactivity: Human, Mouse, Rat, Cow, Horse, Dog, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBFB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CBFB Antibody

Binding Specificity: N-Term

Purpose: CBFB Antibody - N-terminal region

Sequence: CRDGRSEIAF VATGTNLSLQ FFPASWQGEQ RQTPSREYVD LEREAGKVYL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against PEBB. It was validated on Western Blot.

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human PEBB

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP31417-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CBFB

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Alternative Name: CBFB

Background:

Background Information: The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit, it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene.

Gene Name: core-binding factor subunit beta

Alternative Symbols: PEBP2B

Protein Name: core-binding factor subunit beta

Molecular Weight: 20kDa

Gene ID: 865

UniProt: Q13951

Pathways: Regulation of Lipid Metabolism by PPARalpha