MDFI antibody (Middle Region)

Catalog No. ABIN5514974

This anti-MDFI antibody is a Rabbit Polyclonal antibody detecting MDFI in WB. Suitable for Human, Cow, Dog, Pig, Rabbit and Sheep.

Quick Overview for MDFI antibody (Middle Region) (ABIN5514974)

Target: MDFI (MyoD Family Inhibitor (MDFI))

Reactivity: Human, Cow, Dog, Pig, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDFI antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MDFI Antibody

Binding Specificity: Middle Region

Sequence: AVTCQPQGNP LGCTPLLPND SGHPSELGGT RRAGNGALGG PKAHRKLQTH

Predicted Reactivity: Cow: 79%, Dog: 79%, Human: 100%, Pig: 86%, Rabbit: 79%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against MDFI. It was validated on Western Blot.

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human MDFI

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MDFI

Target: MDFI (MyoD Family Inhibitor (MDFI))

Alternative Name: MDFI

Background: This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures.

Alias Symbols: MDFI,

Protein Interaction Partner: PDIA5, USP20, ARID5A, ZNF266, KAT5, SPRY2, SPRY1, NR1H3, ZBTB24, ARHGAP32, KIAA0040, CREB5, MAGED1, ZNF264, GTF3C5, APLN, AKAP17A, ZNF230, ZNF223, ZNF155, ZNF138, ZNF136, ZNF124, ZNF79, WNT11, VHL, NR1H2, TYRO3, TNP2, TNNI1, TGIF1, GNAI2, GATA2, EIF4A2, D

Protein Size: 246

Gene ID: 4188

NCBI Accession: NP_005577

UniProt: Q99750

Pathways: Maintenance of Protein Location