APEX2 antibody (C-Term)

Catalog No. ABIN5515372

The Rabbit Polyclonal anti-APEX2 antibody is suitable to detect APEX2 in samples from Human. It has been validated for WB.

Quick Overview for APEX2 antibody (C-Term) (ABIN5515372)

Target: APEX2 (APEX Nuclease (Apurinic/apyrimidinic Endonuclease) 2 (APEX2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APEX2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-APEX2 Antibody

Binding Specificity: C-Term

Purpose: APEX2 Antibody - C-terminal region

Sequence: LMTPKTPEEK AVAKVVKGQA KTSEAKDEKE LRTSFWKSVL AGPLRTPLCG

Predicted Reactivity: Cow: 85%, Horse: 93%, Human: 100%, Rat: 77%

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human APEX2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP65215-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for APEX2

Target: APEX2 (APEX Nuclease (Apurinic/apyrimidinic Endonuclease) 2 (APEX2))

Alternative Name: APEX2

Background:

Background Information: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Gene Name: apurinic/apyrimidinic endodeoxyribonuclease 2

Alternative Symbols: APE2, XTH2, ZGRF2, APEXL2

Protein Name: DNA-(apurinic or apyrimidinic site) lyase 2

Molecular Weight: 57 kDa

Gene ID: 27301

NCBI Accession: NP_055296

UniProt: Q9UBZ4