HMGCR antibody (C-Term)

Catalog No. ABIN5516965

The Rabbit Polyclonal anti-HMGCR antibody is suitable to detect HMGCR in samples from Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for HMGCR antibody (C-Term) (ABIN5516965)

Target: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGCR antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HMGCR Antibody

Binding Specificity: C-Term

Purpose: HMGCR Antibody - C-terminal region

Sequence: PSIEIGTVGG GTNLLPQQAC LQMLGVQGAC KDNPGENARQ LARIVCGTVM

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Yeast: 100%

Characteristics: This is a rabbit polyclonal antibody against HMDH. It was validated on Western Blot.

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human HMGCR

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP44343-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HMGCR

Target: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Alternative Name: HMGCR

Background:

Background Information: HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene Name: 3-hydroxy-3-methylglutaryl-CoA reductase

Alternative Symbols: LDLCQ3

Protein Name: 3-hydroxy-3-methylglutaryl-coenzyme A reductase

Molecular Weight: 97kDa

Gene ID: 3156

UniProt: P04035

Pathways: AMPK Signaling, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha