HLA-A antibody (C-Term)

Catalog No. ABIN5517133

The Rabbit Polyclonal anti-HLA-A antibody is suitable to detect HLA-A in samples from Human. It has been validated for WB.

Quick Overview for HLA-A antibody (C-Term) (ABIN5517133)

Target: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HLA-A Antibody

Binding Specificity: C-Term

Sequence: QPTIPIVGII AGLVLLGAVI TGAVVAAVMW RRKSSDRKGG SYTQAASSDS

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HLA-A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for HLA-A

Target: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Alternative Name: HLA-A

Background: HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.

Alias Symbols: HLAA

Protein Size: 167

Gene ID: 3105

NCBI Accession: NM_001242758, NP_001229687

UniProt: P30443

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response