NSUN2 antibody (N-Term)

Catalog No. ABIN5517213

This Rabbit Polyclonal antibody specifically detects NSUN2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit, Saccharomyces cerevisiae and Zebrafish (Danio rerio).

Quick Overview for NSUN2 antibody (N-Term) (ABIN5517213)

Target: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSUN2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NSUN2 Antibody

Binding Specificity: N-Term

Purpose: NSUN2 Antibody - N-terminal region

Sequence: LEKFHQFLVS ETESGNISRQ EAVSMIPPLL LNVRPHHKIL DMCAAPGSKT

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 92%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against NSUN2. It was validated on Western Blot.

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human NSUN2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48810-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NSUN2

Target: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Alternative Name: NSUN2

Background:

Background Information: NSUN2 is a RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. NSUN2 methylates cytosine to 5- methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. It may act downstream of Myc to regulate epidermal cell growth and proliferation. And it is required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.

Gene Name: NOP2/Sun RNA methyltransferase family, member 2

Alternative Symbols: MISU, MRT5, SAKI, TRM4

Protein Name: tRNA (cytosine(34)-C(5))-methyltransferase

Molecular Weight: 84kDa

Gene ID: 54888

NCBI Accession: NP_060225

UniProt: Q08J23