Cyclin D1 antibody (N-Term)

Catalog No. ABIN5518654

Product Details anti-Cyclin D1 Antibody

Target: Cyclin D1 (CCND1)

Binding Specificity: AA 19-37, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for G1/S-specific cyclin-D1(CCND1) detection. Tested with WB in Human,Rat.

Sequence: DANLLNDRVL RAMLKAEET

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for G1/S-specific cyclin-D1(CCND1) detection. Tested with WB in Human,Rat.
Gene Name: cyclin D1
Protein Name: G1/S-specific cyclin-D1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Cyclin D1(19-37aa DANLLNDRVLRAMLKAEET), different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for CCND1 is approximately 2.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Cyclin D1 antibody (ABIN5518654)

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Cheng, Wang, Wang, Wang, Du, Lou: "Silencing Ras-Related C3 Botulinum Toxin Substrate 1 Inhibits Growth and Migration of Hypopharyngeal Squamous Cell Carcinoma via the P38 Mitogen-Activated Protein Kinase Signaling Pathway." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 768-781, (2018) (PubMed).

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Xiao, Dong, Cheng, Mao, Zong, Wu, Wang, Xie, Wang, Lei, Guo: "LRIG2 promotes the proliferation and cell cycle progression of glioblastoma cells in vitro and in vivo through enhancing PDGFRβ signaling." in: International journal of oncology, Vol. 53, Issue 3, pp. 1069-1082, (2018) (PubMed).

Ren, Qiu, Lü, Ru, Li, Xiang, Yu, Zhang: "TALENs-directed knockout of the full-length transcription factor Nrf1α that represses malignant behaviour of human hepatocellular carcinoma (HepG2) cells." in: Scientific reports, Vol. 6, pp. 23775, (2017) (PubMed).

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Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: CCND1 (CCND1 Products)

Background: Cyclin D1, also known as CCND1, is a human gene. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1-S phase of the cell cycle. Amplification or overexpression of cyclin D1 plays pivotal roles in the development of a subset of human cancers including parathyroid adenoma, breast cancer, colon cancer, lymphoma, melanoma, and prostate cancer. The cyclin D1 gene is overexpressed in human breast cancers and is required for oncogene-induced tumorigenesis. Brisken et al. (2003) found that prolactin (PRL, 176760) induced IGF2 (147470) mRNA and IGF2 induced cyclin D1 protein expression in mouse mammary epithelial cultures. And they also concluded that IGF2 is a mediator of prolactin-induced alveologenesis and that prolactin, IGF2, and cyclin D1 are components of a developmental pathway in mammary gland.

Synonyms: AI327039 antibody|B cell ccl/lymphoma 1 antibody|B cell CLL/lymphoma 1 antibody|B cell leukemia 1 antibody|B-cell leukemia 1 antibody|B cell lymphoma 1 protein antibody|B-cell lymphoma 1 protein antibody|BCL 1 antibody|BCL-1 antibody|BCL-1 oncogene antibody|BCL1 antibody|BCL1 oncogene antibody|CCND 1 antibody|CCND1 antibody|CCND1 protein antibody|CCND1/FSTL3 fusion gene, included antibody|CCND1/IGHG1 fusion gene antibody|CCND1/IGHG1 fusion gene, included antibody|CCND1/IGLC1 fusion gene, included antibody|CCND1/PTH fusion gene, included antibody|CCND1_HUMAN antibody|cD1 antibody|Cyl 1 antibody|D11S287E antibody|G1/S specific cyclin D1 antibody|G1/S-specific cyclin-D1 antibody|Parathyroid adenomatosis 1 antibody|PRAD1 antibody|PRAD1 oncogene antibody|U21B31 antibody

Gene ID: 595

UniProt: P24385

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling