CD80 antibody (C-Term)

Catalog No. ABIN5518665

Product Details anti-CD80 Antibody

Target: CD80

Binding Specificity: AA 227-245, C-Term

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD80 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for T-lymphocyte activation antigen CD80(CD80) detection. Tested with WB in Rat.

Sequence: DAHVSQNFTW EKPPEDPPD

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for T-lymphocyte activation antigen CD80(CD80) detection. Tested with WB in Rat.
Gene Name: CD80 Molecule
Protein Name: CD80 antigen, isoform CRA_a

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of rat CD80(227-245aa DAHVSQNFTWEKPPEDPPD), different from the related mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, The detection limit for CD80 is approximately 1.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-CD80 antibody (ABIN5518665)

Du, Dong, Zhao, Fu, Wang, Chen, Ou, Li, Sun, Tang, Song: "Immunostimulatory and anti-neoplasm effects of a novel palindrome CpG oligodeoxynucleotide in mice." in: Acta pharmacologica Sinica, Vol. 33, Issue 8, pp. 1047-54, (2012) (PubMed).

Target Details for CD80

Target: CD80

Alternative Name: CD80 (CD80 Products)

Synonyms: B7 antibody, B7-1 antibody, B7.1 antibody, BB1 antibody, CD28LG antibody, CD28LG1 antibody, LAB7 antibody, B71 antibody, Cd28l antibody, Ly-53 antibody, Ly53 antibody, MIC17 antibody, TSA1 antibody, CD80 molecule antibody, Cd80 molecule antibody, CD80 antigen antibody, CD80 antibody, Cd80 antibody

Background: Cluster of Differentiation 80(also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28(for autoregulation and intercellular association) and CTLA-4(for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. Reeves et al.(1997) stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

Synonyms: Activation B7 1 antigen antibody|Activation B7-1 antigen antibody|Activation B71 antigen antibody|B lymphocyte activation antigen B7 antibody|B7 antibody|B7-1 antibody|B7-1 antigen antibody|BB1 antibody|CD 80 antibody|CD28 antigen ligand 1 antibody|CD28 antigen ligand 1 B7 1 antigen antibody|CD28 antigen ligand 1 B71 antigen antibody|CD28LG antibody|CD28LG1 antibody|CD80 antibody|CD80 antigen antibody|CD80 antigen precursor antibody|CD80 Molecule antibody|CD80_HUMAN antibody|Costimulatory factor CD80 antibody|CTLA 4 counter receptor B7.1 antibody|CTLA-4 counter-receptor B7.1 antibody|LAB 7 antibody|LAB7 antibody|T lymphocyte activation antigen CD80 antibody|T-lymphocyte activation antigen CD80 antibody

UniProt: G3V671

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints