HSD11B1 antibody (N-Term)

Catalog No. ABIN5518666

The Rabbit Polyclonal anti-HSD11B1 antibody has been validated for WB and IHC. It is suitable to detect HSD11B1 in samples from Human, Rat, Mouse and Monkey.

Quick Overview for HSD11B1 antibody (N-Term) (ABIN5518666)

Target: HSD11B1 (Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1))

Reactivity: Human, Rat, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD11B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HSD11B1 Antibody

Binding Specificity: AA 16-33, N-Term

Purpose: Anti-HSD11B1 Antibody Picoband®

Sequence: MAYYYYSANE EFRPEMLQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HSD11B1 Antibody (ABIN5518666). Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HSD11B1, different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
1. "Entrez Gene: HSD11B1 hydroxysteroid (11-beta) dehydrogenase 1".

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HSD11B1

Target: HSD11B1 (Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1))

Alternative Name: HSD11B1

Background:

Synonyms: Corticosteroid 11-beta-dehydrogenase isozyme 1,1.1.1.146,11-beta-hydroxysteroid dehydrogenase 1,11-DH,11-beta-HSD1,Short chain dehydrogenase/reductase family 26C member 1,HSD11B1,HSD11, HSD11L, SDR26C1,

Tissue Specificity: Widely expressed. Highest expression in liver.

Background: 11beta-hydroxysteroid dehydrogenase type 1 is an NADPH-dependent enzyme highly expressed in key metabolic tissues including liver, adipose tissue, and the central nervous system. In these tissues, HSD11B1 reduces cortisone to the active hormone cortisol that activates glucocorticoid receptors. It is inhibited by carbenoxolone, a drug typically used in the treatment of peptic ulcers. Tannin et al. (1991) localized the HSD11B1 gene to chromosome 1. The localization was confirmed by isolating the gene from a chromosome 1-specific library using the cDNA as a probe. Schutte et al. (2000) mapped the HSD11B1 gene to 1q32-q41.

Sequence Similarities: Belongs to the connexin family. Beta-type (group I) subfamily.

Molecular Weight: 36 kDa

UniProt: P28845

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Regulation of Carbohydrate Metabolic Process