HSD11B1 antibody (N-Term)

Catalog No. ABIN2773867

There are 2+ publications for this product available. The Rabbit Polyclonal anti-HSD11B1 antibody is suitable to detect HSD11B1 in samples from Human, Rat, Mouse, Dog, Rabbit, Sheep, Cow, Guinea Pig and Horse. It has been validated for WB and IHC.

Quick Overview for HSD11B1 antibody (N-Term) (ABIN2773867)

Target: HSD11B1 (Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1))

Reactivity: Human, Rat, Mouse, Dog, Rabbit, Sheep, Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD11B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HSD11B1 Antibody

Binding Specificity: N-Term

Purpose: HSD11B1 Antibody - N-terminal region

Sequence: QKVVSHCLEL GAASAHYIAG TMEDMTFAEQ FVAQAGKLMG GLDMLILNHI

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Sheep: 93%

Characteristics: This is a rabbit polyclonal antibody against HSD11B1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HSD11B1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP45714-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-HSD11B1 antibody (ABIN2773867)

Kuroda, Venkatakrishnan, Salker, Lucas, Shaheen, Kuroda, Blanks, Christian, Quenby, Brosens: "Induction of 11?-HSD 1 and activation of distinct mineralocorticoid receptor- and glucocorticoid receptor-dependent gene networks in decidualizing human endometrial stromal cells." in: Molecular endocrinology (Baltimore, Md.), Vol. 27, Issue 2, pp. 192-202, (2013) (PubMed).

Lei, Chen, Georgiou, Sooranna, Khanjani, Brosens, Bennett, Johnson: "Progesterone acts via the nuclear glucocorticoid receptor to suppress IL-1?-induced COX-2 expression in human term myometrial cells." in: PLoS ONE, Vol. 7, Issue 11, pp. e50167, (2012) (PubMed).

Target Details for HSD11B1

Target: HSD11B1 (Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1))

Alternative Name: HSD11B1

Background:

Background Information: HSD11B1 is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, HSD11B1 can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children.The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Two transcript variants encoding the same protein have been found for this gene.

Gene Name: Hydroxysteroid (11-beta) dehydrogenase 1

Alternative Symbols: HDL, 11-DH, HSD11, HSD11B, HSD11L, CORTRD2, SDR26C1, 11-beta-HSD1

Protein Name: Corticosteroid 11-beta-dehydrogenase isozyme 1

Molecular Weight: 32kDa

Gene ID: 3290

NCBI Accession: NP_005516

UniProt: P28845

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Regulation of Carbohydrate Metabolic Process