HSD17B1 antibody (N-Term)

Catalog No. ABIN5518679

This anti-HSD17B1 antibody is a Rabbit Polyclonal antibody detecting HSD17B1 in WB, IHC and ICC. Suitable for Human and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for HSD17B1 antibody (N-Term) (ABIN5518679)

Target: HSD17B1 (Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD17B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-HSD17B1 Antibody

Binding Specificity: AA 29-43, N-Term

Purpose: Anti-HSD17B1 Antibody Picoband®

Sequence: QSFKVYATLR DLKTQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HSD17B1 Antibody (ABIN5518679). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HSD17B1, different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunocytochemistry , 0.5-1 μg/mL, Human
1. "Entrez Gene: HSD17B1 Hydroxysteroid (17-beta) dehydrogenase 1". 2. Aka JA, Mazumdar M, Chen CQ, Poirier D, Lin SX (Apr 2010). "17beta-hydroxysteroid dehydrogenase type 1 stimulates breast cancer by dihydrotestosterone inactivation in addition to estradiol production".Molecular Endocrinology. 24 (4): 832-45.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-HSD17B1 antibody (ABIN5518679)

Li, Peng, Zeng, Liu, Hao, Yu, Zhu, Hu: "Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application." in: Scientific reports, Vol. 5, pp. 15286, (2016) (PubMed).

Target Details for HSD17B1

Target: HSD17B1 (Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1))

Alternative Name: HSD17B1

Background:

Synonyms: Estradiol 17-beta-dehydrogenase 1,1.1.1.62,17-beta-hydroxysteroid dehydrogenase type 1,17-beta-HSD 1,20 alpha-hydroxysteroid dehydrogenase,20-alpha-HSD,E2DH,Placental 17-beta-hydroxysteroid dehydrogenase,Short chain dehydrogenase/reductase family 28C member 1,HSD17B1,E17KSR, EDH17B1, EDH17B2, EDHB17, SDR28C1,

Tissue Specificity: Detected in liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, and colon. .

Background: Estradiol 17-beta-dehydrogenase 1 is an enzyme that in humans is encoded by the HSD17B1 gene. This gene encodes a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. The encoded protein catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. This gene is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Polymorphisms in this gene have been linked to breast and prostate cancer. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants.

Sequence Similarities: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Molecular Weight: 37-38 kDa

UniProt: P14061

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis