HSD17B1 antibody (N-Term)

Catalog No. ABIN5518679

Product Details anti-HSD17B1 Antibody

Target: HSD17B1 (Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1))

Binding Specificity: AA 29-43, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD17B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Estradiol 17-beta-dehydrogenase 1(HSD17B1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Sequence: QSFKVYATLR DLKTQ

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Estradiol 17-beta-dehydrogenase 1(HSD17B1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: hydroxysteroid(17-beta) dehydrogenase 1
Protein Name: Estradiol 17-beta-dehydrogenase 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HSD17B1(29-43aa QSFKVYATLRDLKTQ), different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-HSD17B1 antibody (ABIN5518679)

Li, Peng, Zeng, Liu, Hao, Yu, Zhu, Hu: "Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application." in: Scientific reports, Vol. 5, pp. 15286, (2016) (PubMed).

Target Details for HSD17B1

Target: HSD17B1 (Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1))

Alternative Name: HSD17B1 (HSD17B1 Products)

Synonyms: EDH17B2 antibody, EDHB17 antibody, HSD17 antibody, SDR28C1 antibody, 17HSDB1 antibody, 17beta-HSD antibody, E2DH antibody, Hsd17ba antibody, 17BHD1 antibody, zfHSD17B1 antibody, hydroxysteroid 17-beta dehydrogenase 1 antibody, hydroxysteroid (17-beta) dehydrogenase 1 antibody, HSD17B1 antibody, Hsd17b1 antibody, hsd17b1 antibody

Background: Estradiol 17-beta-dehydrogenase 1 is an enzyme that in humans is encoded by the HSD17B1 gene. This gene encodes a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. The encoded protein catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. This gene is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Polymorphisms in this gene have been linked to breast and prostate cancer. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants.

Synonyms: 17 beta HSD 1 antibody|17 beta hydroxysteroid dehydrogenase type 1 antibody|17-beta-HSD 1 antibody|17-beta-hydroxysteroid dehydrogenase type 1 antibody|20 alpha-hydroxysteroid dehydrogenase antibody|20-alpha-HSD antibody|DHB1_HUMAN antibody|E17KSR antibody|E2DH antibody|EDH17B1 antibody|EDH17B2 antibody|EDHB17 antibody|Estradiol 17 beta dehydrogenase 1 antibody|Estradiol 17-beta-dehydrogenase 1 antibody|HSD17 antibody|HSD17B1 antibody|Hydroxysteroid(17 beta) dehydrogenase 1 antibody|MGC13814 antibody|Placental 17 beta hydroxysteroid dehydrogenase antibody|Placental 17-beta-hydroxysteroid dehydrogenase antibody|SDR28C1 antibody|Short chain dehydrogenase/reductase family 28CE,member 1 antibody

UniProt: P14061

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis