C5 antibody (C-Term)

Catalog No. ABIN5518715

This anti-C5 antibody is a Rabbit Polyclonal antibody detecting C5 in WB. Suitable for Rat.

Quick Overview for C5 antibody (C-Term) (ABIN5518715)

Target: C5 (Complement Component 5 (C5))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-C5 Antibody

Binding Specificity: AA 59-77, C-Term

Purpose: Anti-C5/C5a Antibody Picoband®

Sequence: TIADKIRKES HHKGMLLGR

Cross-Reactivity (Details): No cross reactivity with other proteins

Characteristics: Rabbit IgG polyclonal antibody for Complement C5(C5) detection. Tested with WB in Rat.
Gene Name: complement component 5
Protein Name: Complement C5

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of rat C5a, different from the related mouse sequence by five amino acids.

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Rat
1. Delgado-Cervino, E., Fontan, G., Lopez-Trascara, M. C5 complement deficiency in a Spanish family: molecular characterization of the double mutation responsible for the defect. Molec. Immun. 42: 105-111, 2005. 2. Pfarr, N., Prawitt, D., Kirschfink, M., Schroff, C., Knuf, M., Habermehl, P., Mannhardt, W., Zepp, F., Fairbrother, W. G., Loos, M., Burge, C. B., Pohlenz, J. Linking C5 deficiency to an exonic splicer enhancer mutation. J. Immun. 174: 4172-4177, 2005. Note: Erratum: J. Immun. 182: 5152 only, 2009.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for C5

Target: C5 (Complement Component 5 (C5))

Alternative Name: C5

Background:

Synonyms: Complement C5,C5a anaphylatoxin,C5,

Tissue Specificity: Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. .

Background: C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.

Sequence Similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

Molecular Weight: 42 kDa

Gene ID: 362119

UniProt: P08650

Pathways: Complement System, Carbohydrate Homeostasis