CYGB antibody (AA 1-190)

Catalog No. ABIN5518753

The Rabbit Polyclonal anti-CYGB antibody is suitable to detect CYGB in samples from Human, Rat and Mouse. It has been validated for WB and IHC.

Quick Overview for CYGB antibody (AA 1-190) (ABIN5518753)

Target: CYGB (Cytoglobin (CYGB))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYGB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CYGB Antibody

Binding Specificity: AA 1-190

Purpose: Anti-Cytoglobin/CYGB Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human Cytoglobin shares 95.3%,93.7% amino acid (aa) sequence identity with mouse,rat Cytoglobin,respectively.

Characteristics: Anti-Cytoglobin/CYGB Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Cytoglobin recombinant protein (Position: M1-P190). Human Cytoglobin shares 95.3% and 93.7% amino acid (aa) sequence identity with mouse and rat Cytoglobin, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CYGB

Target: CYGB (Cytoglobin (CYGB))

Alternative Name: CYGB

Background:

Background: Cytoglobin (CYGB), also called HGB or STAP, is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress. The cytoglobin gene is mapped on 17q25.1. The CYGB gene contains 4 exons and spans about 9 kb. Cytoglobin has many elements common to vertebrate globins, including invariant histidine residues, and the amino acids that form the heme pocket share similarity with pentacoordinate myoglobin. In contrast to the high oxygen affinities displayed by most hexacoordinate hemoglobins, the characteristics of CYGB indicate that it can facilitate oxygen transport. Because the oxygen affinity of CYGB is more similar to myoglobin than to neuroglobin, and the oxy form of CYGB resists autooxidation, CYGB is proposed to represent a tissue oxygen reservoir.

Gene Full Name: cytoglobin

Molecular Weight: 21 kDa

Gene ID: 114757

UniProt: Q8WWM9