TUB antibody (C-Term)

Catalog No. ABIN5518797

This Rabbit Polyclonal antibody specifically detects TUB in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for TUB antibody (C-Term) (ABIN5518797)

Target: TUB (Tubby Homolog (TUB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUB antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-TUB Antibody

Binding Specificity: AA 395-429, C-Term

Purpose: Anti-TUB 1 Antibody Picoband®

Sequence: VHERVSIRPR NEHETLLARW QNKNTESIIE LQNKT

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TUB 1 Antibody Picoband® (ABIN5518797). Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TUB 1, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: TUB tubby homolog (mouse)". 2. Kapeller, R, Moriarty A, Strauss A, Stubdal H, Theriault K, Siebert E, Chickering T, Morgenstern J P, Tartaglia L A, Lillie J (Aug 1999). "Tyrosine phosphorylation of tub and its association with Src homology 2 domain-containing proteins implicate tub in intracellular signaling by insulin". J. Biol. Chem. UNITED STATES. 274 (35): 24980-6. 3. Kleyn PW, Fan W, Kovats SG, Lee JJ, Pulido JC, Wu Y, Berkemeier LR, Misumi DJ, Holmgren L, Charlat O, Woolf EA, Tayber O, Brody T, Shu P, Hawkins F, Kennedy B, Baldini L, Ebeling C, Alperin GD, Deeds J, Lakey ND, Culpepper J, Chen H, Glucksmann-Kuis MA, Carlson GA, Duyk GM, Moore KJ (Jun 1996). "Identification and characterization of the mouse obesity gene tubby: a member of a novel gene family". Cell. 85 (2): 281-90.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TUB

Target: TUB (Tubby Homolog (TUB))

Alternative Name: TUB

Background:

Synonyms: Tubby protein homolog,TUB,

Tissue Specificity: Ubiquitous.

Background: Tubby protein homolog is a protein that in humans is encoded by the TUB gene. This gene encodes a member of the Tubby family of bipartite transcription factors. The encoded protein may play a role in obesity and sensorineural degradation. The crystal structure has been determined for a similar protein in mouse, and it functions as a membrane-bound transcription regulator that translocates to the nucleus in response to phosphoinositide hydrolysis. Two transcript variants encoding distinct isoforms have been identified for this gene.

Molecular Weight: 62 kDa

Gene ID: 7275

UniProt: P50607

Pathways: RTK Signaling, Sensory Perception of Sound