CYP2D6 antibody (C-Term)

Catalog No. ABIN5518823

This anti-CYP2D6 antibody is a Rabbit Polyclonal antibody detecting CYP2D6 in WB and IHC. Suitable for Human, Rat and Mouse.

Quick Overview for CYP2D6 antibody (C-Term) (ABIN5518823)

Target: CYP2D6 (Cytochrome P450, Family 2, Subfamily D, Polypeptide 6 (CYP2D6))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP2D6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CYP2D6 Antibody

Binding Specificity: AA 315-347, C-Term

Purpose: Anti-Cytochrome P450 2D6/CYP2D6 Antibody Picoband®

Sequence: AWGLLLMILH PDVQRRVQQE IDDVIGQVRR PEM

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Cytochrome 2D6/CYP2D6 Antibody Picoband® (ABIN5518823). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cytochrome P450 2D6.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1.""Entrez Gene: CYP2D6 cytochrome P450, family 2, subfamily D, polypeptide 6". 2. Distlerath, L. M., Guengerich, F. P.Characterization of a human liver cytochrome P-450 involved in the oxidation of debrisoquine and other drugs by using antibodies raised to the analogous rat enzyme.Proc. Nat. Acad. Sci.81: 7348-7352, 1984. 3. Gonzalez, F. J., Matsunaga, T., Nagata, K., Meyer, U. A., Nebert, D. W., Pastewka, J., Kozak, C. A., Gillette, J., Gelboin, H. V., Hardwick, J. P.Debrisoquine 4-hydroxylase: characterization of a new P450 gene subfamily, regulation, chromosomal mapping, and molecular analysis of the DA rat polymorphism.DNA6: 149-161, 1987.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CYP2D6

Target: CYP2D6 (Cytochrome P450, Family 2, Subfamily D, Polypeptide 6 (CYP2D6))

Alternative Name: CYP2D6

Background:

Synonyms: Cytochrome P450 2D6,1.14.14.1,CYPIID6,Cytochrome P450-DB1,Debrisoquine 4-hydroxylase,CYP2D6,CYP2DL1,

Tissue Specificity: Widely expressed in hematopoietic cells. Expressed in neutrophils. Within the B-cell compartment, expressed from pro- and pre-B cells to plasma cells.

Background: Cytochrome P450 2D6 (CYP2D6) is one of the most important enzymes involved in the metabolism of xenobiotics in the body. It is a member of Cytochrome P450, family 2, subfamily D, polypeptide 6. This gene is mapped to chromosome 22q13.1. It has got 497 amino acid proteins which shares 73 % sequence identity with the rat protein. CYP2D6 is highly expressed in human liver and it is the major isozyme involved in the formation of N-hydroxyprocainamide, a metabolite potentially involved in the drug-induced lupus syndrome observed with procainamide.

Molecular Weight: 56 kDa

Gene ID: 1565

UniProt: P10635