MAP2K6 antibody (AA 280-334)

Catalog No. ABIN5518934

Product Details anti-MAP2K6 Antibody

Target: MAP2K6 (Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6))

Binding Specificity: AA 280-334

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2K6 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Anti-MEK6/MAP2K6 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MEK6/MAP2K6 Antibody Picoband® (ABIN5518934). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human MEK6 recombinant protein (Position: A280-D334). Human MEK6 shares 92.7% amino acid (aa) sequence identity with mouse MEK6.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Han, J., Lee, J.-D., Jiang, Y., Li, Z., Feng, L., Ulevitch, R. J. Characterization of the structure and function of a novel MAP kinase kinase (MKK6). J. Biol. Chem. 271: 2886-2891, 1996. 2. Mao, Z., Bonni, A., Xia, F., Nadal-Vicans, M., Greenberg, M. E. Neuronal activity-dependent cell survival mediated by transcription factor MEF2. Science 286: 785-790, 1999. 3. Mukherjee, S., Keitany, G., Li, Y., Wang, Y., Ball, H. L., Goldsmith, E. J., Orth, K. Yersinia YopJ acetylates and inhibits kinase activation by blocking phosphorylation. Science 312: 1211-1214, 2006.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MAP2K6

Target: MAP2K6 (Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6))

Alternative Name: MAP2K6 (MAP2K6 Products)

Background:

Synonyms: Dual specificity mitogen-activated protein kinase kinase 6,MAP kinase kinase 6,MAPKK 6,2.7.12.2,MAPK/ERK kinase 6,MEK 6,Stress-activated protein kinase kinase 3,SAPK kinase 3,SAPKK-3,SAPKK3,MAP2K6,MEK6, MKK6, PRKMK6, SKK3,

Tissue Specificity: Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas. .

Background: MAP2K6 (Mitogen-activated protein kinase kinase 6), also known as MAP kinase kinase 6 (MAPKK 6) or MAPK/ERK kinase 6 is an enzyme that in humans is encoded by the MAP2K6 gene. It is located on chromosome 17. MAPKK 6 is a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediatedsignal transduction pathway, this gene is involved in many cellular processes such as stress-induced cell cycle arrest, transcription activation and apoptosis.

Molecular Weight: 38 kDa

Gene ID: 5608

UniProt: P52564

Pathways: MAPK Signaling, TLR Signaling, Activation of Innate immune Response, Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades