Endoglin antibody (AA 27-226)

Catalog No. ABIN5518982

Cited in 7+ publications. The Rabbit Polyclonal anti-Endoglin antibody (ABIN5518982) specifically detects Endoglin in WB, IHC and ELISA. The antibody is reactive with Mouse and Rat samples.

Quick Overview for Endoglin antibody (AA 27-226) (ABIN5518982)

Target: Endoglin (ENG)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endoglin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Endoglin Antibody

Binding Specificity: AA 27-226

Purpose: Anti-CD105/Eng Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-/Eng Antibody (ABIN5518982). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse CD105 recombinant protein (Position: E27-L226).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Endoglin antibody (ABIN5518982)

Ding, Chang, Niu, Dai, Geng, Li, Guo, Xu: "Overexpression of transcription factor Foxa2 and Hnf1α induced rat bone mesenchymal stem cells into hepatocytes." in: Cytotechnology, Vol. 68, Issue 5, pp. 2037-47, (2016) (PubMed).

Zhang, Shang, Hao, Zheng, Li, Liang, Cui, Liu: "Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats." in: American journal of translational research, Vol. 7, Issue 11, pp. 2176-86, (2016) (PubMed).

Yuan, Liu, Li, Li, Sun, Xu, Man, Fu: "Effects of BMSCs interactions with adventitial fibroblasts in transdifferentiation and ultrastructure processes." in: International journal of clinical and experimental pathology, Vol. 7, Issue 7, pp. 3957-65, (2015) (PubMed).

Li, Zeng, Qi, Tang, Zhang, Wu, Liang, Shi, Liu, Zhang: "Xenotransplantation of human adipose-derived stem cells in zebrafish embryos." in: PLoS ONE, Vol. 10, Issue 4, pp. e0123264, (2015) (PubMed).

Liu, Feng, Dong, Yang, Li, Chen, Zhang, Wang, Zhou, Zhao: "Administration of BMSCs with muscone in rats with gentamicin-induced AKI improves their therapeutic efficacy." in: PLoS ONE, Vol. 9, Issue 5, pp. e97123, (2014) (PubMed).

Dai, Li, Zhou, Chen, Chen, Xiao: "Genistein promotion of osteogenic differentiation through BMP2/SMAD5/RUNX2 signaling." in: International journal of biological sciences, Vol. 9, Issue 10, pp. 1089-98, (2013) (PubMed).

Ren, Ren, Zhao, Wang, Zuo, Yu: "Antitumor activity of endogenous mFlt4 displayed on a T4 phage nanoparticle surface." in: Acta pharmacologica Sinica, Vol. 30, Issue 5, pp. 637-45, (2009) (PubMed).

Target Details for Endoglin

Target: Endoglin (ENG)

Alternative Name: Eng

Background:

Synonyms: Endoglin, Cell surface MJ7/18 antigen, CD105, Eng, Edg,

Background: Endoglin (Osler-Rendu-Weber syndrome 1), CD105, is a type I membrane glycoprotein located on cell surfaces and is a part of the TGF beta receptor complex. Its gene is mapped to human chromosome 8. The protein consists of a homodimer of 180 kDA with disulfide links. It has been found on endothelial cells, activated macrophages, fibroblasts and smooth muscle cells. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling and has been found to be elevated in pregnant women who subsequently develop preeclampsia.

Molecular Weight: 95-105 kDa

Gene ID: 13805

UniProt: Q63961