Endoglin (ENG) antibody

Details for Product No. ABIN93943
Antigen
Reactivity
Human, Pig (Porcine)
455
174
83
18
14
8
5
5
5
4
1
Host
Mouse
260
249
47
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
56
52
46
22
8
8
7
7
7
7
7
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
352
340
130
115
80
72
47
38
26
20
17
13
12
7
7
5
4
4
3
2
2
2
2
1
Options
Immunogen Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA.
Clone MEM-229
Isotype IgG2a
Specificity The antibody MEM-229 recognizes CD105 (Endoglin), a 90 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow, it is also present on syncytiotrophoblast on placenta throughout pregnancy.
No Cross-Reactivity Dog (Canine), Horse (Equine)
Cross-Reactivity (Details) Human, Porcine
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD105 (ENG Antibody Abstract)
Background CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Endoglin (ENG) antibody (ABIN93943) Immunofluorescence staining of an infarcted porcine heart with anti-CD105 (MEM-229 ;
Product cited in: Plánka, Necas, Srnec, Rauser, Starý, Jancár, Amler, Filová, Hlucilová, Kren, Gál: "Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs." in: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 58, Issue 6, pp. 885-93, 2010 (PubMed).