MED13 antibody (AA 61-240)

Catalog No. ABIN5519033

This anti-MED13 antibody is a Rabbit Polyclonal antibody detecting MED13 in ELISA, WB, FACS, ICC and IF. Suitable for Human and Mouse.

Quick Overview for MED13 antibody (AA 61-240) (ABIN5519033)

Target: MED13 (Mediator Complex Subunit 13 (MED13))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED13 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-MED13 Antibody

Binding Specificity: AA 61-240

Purpose: Anti-MED13 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MED13 Antibody Picoband® (ABIN5519033). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human MED13 recombinant protein (Position: L61-Y240).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Gustafsson CM, Samuelsson T (Jul 2001). "Mediator--a universal complex in transcriptional regulation". Molecular Microbiology. 41 (1): 1-8. 2. Tuchin DA, Erve PR, Schumer W (1976). "Prevention of endotoxin--induced irreversible platelet aggregation in vitro". Surgical Forum. 27 (62): 22-3.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MED13

Target: MED13 (Mediator Complex Subunit 13 (MED13))

Alternative Name: MED13

Background:

Synonyms: Mediator of RNA polymerase II transcription subunit 13, Activator-recruited cofactor 250 kDa component, ARC250, Mediator complex subunit 13, Thyroid hormone receptor-associated protein 1, Thyroid hormone receptor-associated protein complex 240 kDa component, Trap240, Vitamin D3 receptor-interacting protein complex component DRIP250, DRIP250, MED13, ARC250, KIAA0593, THRAP1, TRAP240,

Tissue Specificity: Ubiquitous.

Background: Mediator complex subunit 13 is a protein that in humans is encoded by the MED13 gene. This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.

Molecular Weight: 300 kDa

Gene ID: 9969

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha