MED13 antibody (AA 650-730)

Catalog No. ABIN7226192

The Rabbit Polyclonal anti-MED13 antibody (ABIN7226192) specifically detects MED13 in ELISA, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for MED13 antibody (AA 650-730) (ABIN7226192)

Target: MED13 (Mediator Complex Subunit 13 (MED13))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED13 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MED13 Antibody

Binding Specificity: AA 650-730

Purpose: Rabbit Anti-TRAP240 Polyclonal Antibody

Specificity: TRAP240 Polyclonal Antibody detects endogenous levels of TRAP240 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human TRAP240 at AA range: 650-730

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MED13

Target: MED13 (Mediator Complex Subunit 13 (MED13))

Alternative Name: TRAP240

Background: MED13, ARC250, KIAA0593, THRAP1, TRAP240, Mediator of RNA polymerase II transcription subunit 13, Activator-recruited cofactor 250 kDa component, ARC250, Mediator complex subunit 13, Thyroid hormone receptor-associated protein 1, Thyroid hoMED13 (mediator complex subunit 13) encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of MED13 is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.

Gene ID: 9969

UniProt: Q9UHV7

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha