MED20 antibody (AA 1-212)

Catalog No. ABIN5519037

Product Details anti-MED20 Antibody

Target: MED20 (Mediator Complex Subunit 20 (MED20))

Binding Specificity: AA 1-212

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED20 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for TRFP / MED20 detection. Tested with WB, Direct ELISA in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for TRFP / MED20 detection. Tested with WB, Direct ELISA in Human,Mouse,Rat.
Gene Name: mediator complex subunit 20
Protein Name: Mediator of RNA polymerase II transcription subunit 20

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived huamn TRFP / MED20 recombinant protein (Position: M1-R212).

Isotype: IgG

Application Details

Application Notes: Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for MED20

Target: MED20 (Mediator Complex Subunit 20 (MED20))

Alternative Name: MED20 (MED20 Products)

Synonyms: PRO0213 antibody, TRFP antibody, 1110011O05Rik antibody, 2410115I17Rik antibody, AU018348 antibody, Trfp antibody, Usp49 antibody, trfp antibody, zgc:101043 antibody, mediator complex subunit 20 antibody, mediator complex subunit 20 S homeolog antibody, MED20 antibody, Med20 antibody, med20 antibody, med20.S antibody

Background: MED20 is a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder.

Synonyms: Mediator of RNA polymerase II transcription subunit 20, Mediator complex subunit 20, TRF-proximal protein homolog, hTRFP, MED20, TRFP

Gene ID: 9477

UniProt: Q9H944

Pathways: Regulation of Lipid Metabolism by PPARalpha