NG2 antibody

Catalog No. ABIN5526901

The Mouse Monoclonal anti-NG2 antibody (Clone 7-1) (ABIN5526901) specifically detects NG2 in WB, IP, ICC and FACS. The antibody is reactive with Human samples.

Quick Overview for NG2 antibody (ABIN5526901)

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 7-1

Product Details anti-NG2 Antibody

Purpose: Anti-NG2 Purified

Cross-Reactivity (Details): Human

Characteristics: The mouse monoclonal antibody 7.1 recognizes an extracellular epitope of NG2, the melanoma-associated chondroitin sulfate proteoglycan 4 of Mw approximately 220-300 kDa.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human bone marrow stromal cells infected with SV-40

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for NG2

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Alternative Name: NG2

Background: Chondroitin sulfate proteoglycan 4,NG2 / chondroitin sulfate proteoglycan 4 is expressed on glial cell populations, but not on normal hepatopoietic cells. It is an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells, where it plays role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes, and supports signaling pathways important for tumor invasion and growth. NG2 also serves as an AML blast tumor marker associated with poor prognosis.,Chondroitin sulfate proteoglycan 4, MSK16, CSPG4, MCSP

Gene ID: 1464

UniProt: Q6UVK1

Pathways: Glycosaminoglycan Metabolic Process