PRKAG3 antibody (AA 265-295)

Catalog No. ABIN5533705

The Rabbit Polyclonal anti-PRKAG3 antibody (ABIN5533705) specifically detects PRKAG3 in WB and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for PRKAG3 antibody (AA 265-295) (ABIN5533705)

Target: PRKAG3 (Protein Kinase, AMP-Activated, gamma 3 Non-Catalytic Subunit (PRKAG3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAG3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PRKAG3 Antibody

Binding Specificity: AA 265-295

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Immunogen: This AMPK gamma (PRKAG1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-295 amino acids from the Central region of human AMPK gamma (PRKAG1).

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PRKAG3

Target: PRKAG3 (Protein Kinase, AMP-Activated, gamma 3 Non-Catalytic Subunit (PRKAG3))

Alternative Name: AMPK gamma

Background: PRKAG1 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK.

Molecular Weight: 38 kDa

Gene ID: 5571

UniProt: P54619

Pathways: AMPK Signaling, Cellular Glucan Metabolic Process, Warburg Effect