POLG antibody (C-Term)

Catalog No. ABIN5534169

The Rabbit Polyclonal anti-POLG antibody has been validated for WB, IHC (p), IF and FACS. It is suitable to detect POLG in samples from Human and Mouse.

Quick Overview for POLG antibody (C-Term) (ABIN5534169)

Target: POLG (Polymerase (DNA Directed), gamma (POLG))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-POLG Antibody

Binding Specificity: AA 1120-1148, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This POLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1120-1148 amino acids from the C-terminal region of human POLG.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.46 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for POLG

Target: POLG (Polymerase (DNA Directed), gamma (POLG))

Alternative Name: POLG

Background: Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene.

Molecular Weight: 140 kDa

Gene ID: 5428

UniProt: P54098