Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Immunogen
This PARP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-214 amino acids from the N-terminal region of human PARP1.
PARP1
Reactivity: Human
WB, ELISA, IHC, FACS, IF
Host: Rabbit
Monoclonal
8C7
unconjugated
Recombinant Antibody
Application Notes
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Restrictions
For Research Use only
Format
Liquid
Concentration
2 mg/mL
Buffer
Supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PARP1 is a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.