HSPD1 antibody (AA 333-344)

Catalog No. ABIN5539538

This Goat Polyclonal antibody specifically detects HSPD1 in WB, IHC, IF and ELISA. It exhibits reactivity toward Human, Rat and Pig.

Quick Overview for HSPD1 antibody (AA 333-344) (ABIN5539538)

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Reactivity: Human, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This HSPD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Grade: Verified

Product Details anti-HSPD1 Antibody

Binding Specificity: AA 333-344

Purpose: HSP60 / HSPD1 (aa333-344)

Sequence: NLEDVQPHDL GK

Specificity: Reported variants represent identical protein: NP_955472.1, NP_002147.2.

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 60 kDa band observed in Human and Pig Heart, Kidney and Liver and in Rat Kidney lysates (calculated MW of 61.1 kDa according to Human NP_002147.2 and 60.9 kDa according to Pig NP_001241645.1 and Rat NP_071565.2 ). Recommended concentrat

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the mitochondria of HeLa cells and in the cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HSPD1

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Alternative Name: HSPD1

Background: HSPD1, heat shock 60 kDa protein 1 (chaperonin), CPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13, 60 kDa chaperonin, 60 kDa heat shock protein, mitochondrial, P60 lymphocyte protein, chaperonin 60, heat shock protein 65, mitochondrial matrix prote

Gene ID: 3329

NCBI Accession: NP_002147

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity