Paired Box 3 antibody (Internal Region)

Catalog No. ABIN5539569

The Goat Polyclonal anti-Paired Box 3 antibody has been validated for WB and ELISA. It is suitable to detect Paired Box 3 in samples from Human, Mouse and Rat.

Quick Overview for Paired Box 3 antibody (Internal Region) (ABIN5539569)

Target: Paired Box 3 (PAX3)

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Paired Box 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Paired Box 3 Antibody

Binding Specificity: Internal Region

Purpose: PAX3

Sequence: STVHQSTIPS NPD

Specificity: This antibody is expected to recognize isoforms PAX3 (NP_852122.1), PAX3d (NP_852123.1), PAX3e (NP_852124.1), PAX3g (NP_852126.1), PAX3h (NP_852125.1), and PAX3i (NP_001120838.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in Human Brain (Frontal Cortex) and Mouse and Rat Brain lysates (calculated MW of 53.0 kDa according to Human NP_852122.1 and 52.9 kDa according to Mouse NP_032807.3). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Paired Box 3

Target: Paired Box 3 (PAX3)

Alternative Name: PAX3

Background: PAX3, paired box 3, CDHS, HUP2, MGC120381, MGC120382, MGC120383, MGC120384, MGC134778, WS1, paired box homeotic gene 3, paired domain gene 3, paired domain gene HuP2

Gene ID: 5077, 18505, 114502

NCBI Accession: NP_852122, NP_000429, NP_039230, NP_852123, NP_852124, NP_852126, NP_852125, NP_001120838

Pathways: Sensory Perception of Sound, Tube Formation