PKC mu antibody (AA 383-395)

Catalog No. ABIN5539576

This anti-PKC mu antibody is a Goat Polyclonal antibody detecting PKC mu in WB, ELISA and IP. Suitable for Human.

Quick Overview for PKC mu antibody (AA 383-395) (ABIN5539576)

Target: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PKC mu antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Grade: Verified

Product Details anti-PKC mu Antibody

Binding Specificity: AA 383-395

Purpose: PRKD1 (aa383-395)

Sequence: QDPDPDHEDA NRT

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: In transfected HEK293 transiently expressing Human PKD1 (DYKDDDDK tagged), a band of approx. 100 kDa is observed. The same band is observed using anti- DYKDDDDK antibody and no bands are observed in cells transiently expressing Human PKD2 or

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunoprecipitation: PKD1 was immunoprecipitated from Hek293T cells (as above). Immunoprecipitates were analyzed by Western Blot probed with the same antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PKC mu

Target: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Alternative Name: PRKD1

Background: PRKD1, PKC-MU, PKCM, PKD, PRKCM,

Gene ID: 5587

NCBI Accession: NP_002733

Pathways: Myometrial Relaxation and Contraction