S100B antibody (Internal Region)

Catalog No. ABIN5539587

The Goat Polyclonal anti-S100B antibody is suitable to detect S100B in samples from Human. It has been validated for WB, IHC and ELISA.

Quick Overview for S100B antibody (Internal Region) (ABIN5539587)

Target: S100B (S100 Calcium Binding Protein B (S100B))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This S100B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-S100B Antibody

Binding Specificity: Internal Region

Purpose: S100B

Sequence: SHFLEEIKEQ EVVD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5 μg/mL.

Western Blot: Approx 10 kDa band observed in Human Brain (Olfactory Bulb and Cerebellum) lysates (calculated MW of 10.7 kDa according to NP_006263.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for S100B

Target: S100B (S100 Calcium Binding Protein B (S100B))

Alternative Name: S100B

Background: S100B, S100 calcium binding protein B, NEF, S100, S100-B, S100beta, S-100 calcium-binding protein, beta chain, S-100 protein subunit beta, S100 calcium-binding protein, beta (neural), protein S100-B

Gene ID: 6285

NCBI Accession: NP_006263

Pathways: Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins