JAG1 antibody (C-Term)

Catalog No. ABIN5539629

The Goat Polyclonal anti-JAG1 antibody is suitable to detect JAG1 in samples from Human and Mouse. It has been validated for IHC, ELISA and IF.

Quick Overview for JAG1 antibody (C-Term) (ABIN5539629)

Target: JAG1 (Jagged 1 (JAG1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This JAG1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-JAG1 Antibody

Binding Specificity: C-Term

Purpose: JAG1

Sequence: TNKQDNRDLE SAQS

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows membrane staining in the epithelial cells of convoluted tubules. Recommended concentration, 2-4 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 5μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for JAG1

Target: JAG1 (Jagged 1 (JAG1))

Alternative Name: JAG1

Background: JAG1, jagged 1, AGS, AHD, AWS, CD339, HJ1, JAGL1, MGC104644, protein jagged-1

Gene ID: 182, 16449, 29146

NCBI Accession: NP_000205

Pathways: Notch Signaling, Stem Cell Maintenance