CD247 antibody (Internal Region)

Catalog No. ABIN5539696

Product Details anti-CD247 Antibody

Target: CD247 (CD247 Molecule (CD247))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD247 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: CD3-ZETA / CD247

Sequence: KNPQEGLYNE LQKD

Specificity: This antibody is expected to recognize both reported isoforms (NP_932170.1, NP_000725.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 16-17 kDa band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 18.6 kDa according to NP_000725.1). Recommended concentration: 0.1-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CD247

Target: CD247 (CD247 Molecule (CD247))

Alternative Name: CD247 (CD247 Products)

Synonyms: CD3-ZETA antibody, CD3H antibody, CD3Q antibody, CD3Z antibody, T3Z antibody, TCRZ antibody, 4930549J05Rik antibody, A430104F18Rik antibody, AW552088 antibody, Cd3 antibody, Cd3-eta antibody, Cd3-zeta antibody, Cd3h antibody, Cd3z antibody, Cd3zeta antibody, T3z antibody, Tcrk antibody, Tcrz antibody, LOC100136514 antibody, LOC100136549 antibody, LOC100305023 antibody, CD247 antibody, TCRzeta antibody, CD247 molecule antibody, CD247 antigen antibody, CD3zeta-2 antibody, CD3zeta-1 antibody, Cd247 molecule antibody, CD247 antibody, Cd247 antibody, cd3z antibody, cd247 antibody

Background: CD247, CD247 Molecule, CD3-ZETA, CD3H, CD3Q, CD3Z, T3Z, TCRZ, CD247 antigen, zeta subunit, CD3Z antigen, zeta polypeptide (TiT3 complex), CD3zeta chain, T-cell antigen receptor complex, zeta subunit of CD3, T-cell receptor T3 zeta chain, T-cell surface gl

Gene ID: 919

NCBI Accession: NP_932170, NP_000725

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway