DBH antibody (AA 437-448)

Catalog No. ABIN5539717

The Goat Polyclonal anti-DBH antibody (ABIN5539717) specifically detects DBH in WB, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for DBH antibody (AA 437-448) (ABIN5539717)

Target: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DBH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-DBH Antibody

Binding Specificity: AA 437-448

Purpose: DBH (aa437-448)

Sequence: DNHYSPHFQE IR

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Adrenal Gland. Recommended concentration: 5 μg/mL.

Western Blot: Approx 70 kDa band observed in lysates of cell line Jurkat (calculated MW of 69.1 kDa according to NP_000778.3). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat Brain f

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DBH

Target: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Alternative Name: DBH

Background: DBH, dopamine beta-hydroxylase (dopamine beta-monooxygenase), DBM, dopamine beta-hydroxylase

Gene ID: 1621, 13166, 25699

NCBI Accession: NP_000778

Pathways: Carbohydrate Homeostasis