IDE antibody (Internal Region) (Biotin)

Catalog No. ABIN5539736

This Goat Polyclonal antibody specifically detects IDE in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for IDE antibody (Internal Region) (Biotin) (ABIN5539736)

Target: IDE (Insulin-Degrading Enzyme (IDE))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IDE antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-IDE Antibody (Biotin)

Binding Specificity: Internal Region

Purpose: Insulysin / Insulinase, Biotinylated

Sequence: CQQYNFDRDN TE.

Specificity: This antibody is expected to recognize all reported isoforms (NP_004960.2, NP_001159418.1, NP_001309722.1, NP_001309723.1, NP_001309724.1, NP_001309726.1). Reported variants represent identical protein: NP_001309725.1, NP_001309724.1

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 120 kDa band observed in lysates of cell line K562 (calculated MW of 118 kDa according to NP_004960.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IDE

Target: IDE (Insulin-Degrading Enzyme (IDE))

Alternative Name: IDE

Background: IDE, insulin degrading enzyme, INSULYSIN, Abeta-degrading protease, insulin protease, insulinase

Gene ID: 3416

NCBI Accession: NP_004960, NP_001159418, NP_001309722, NP_001309723, NP_001309724, NP_001309726

Pathways: SARS-CoV-2 Protein Interactome