PRKACA antibody (Internal Region)

Catalog No. ABIN5539751

This Goat Polyclonal antibody specifically detects PRKACA in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for PRKACA antibody (Internal Region) (ABIN5539751)

Target: PRKACA (Protein Kinase A, alpha (PRKACA))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PRKACA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PRKACA Antibody

Binding Specificity: Internal Region

Purpose: PRKACA

Sequence: ESPAQNTAHL DQ

Specificity: This antibody is expected to recognize all three reported isoforms (NP_002721.1, NP_997401.1, NP_001291278.1). Based on the choice of the immunizing peptide there is no expected cross-reactivity with the other subunits.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48 kDa band observed in lysates of cell line HeLa and MCF7 (calculated MW of 49.1 kDa according to NP_002721.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PRKACA

Target: PRKACA (Protein Kinase A, alpha (PRKACA))

Alternative Name: PRKACA

Background: PRKACA, protein kinase, cAMP-dependent, catalytic, alpha, PKACA, PPNAD4, PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha, protein kinase A catalytic subunit

Gene ID: 5566

NCBI Accession: NP_002721, NP_997401, NP_001291278

Pathways: NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, Lipid Metabolism, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection