GLP1R antibody (Internal Region)

Catalog No. ABIN5539773

The Goat Polyclonal anti-GLP1R antibody is suitable to detect GLP1R in samples from Human and Mouse. It has been validated for WB, ELISA and IHC.

Quick Overview for GLP1R antibody (Internal Region) (ABIN5539773)

Target: GLP1R (Glucagon-Like Peptide 1 Receptor (GLP1R))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This GLP1R antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-GLP1R Antibody

Binding Specificity: Internal Region

Purpose: GLP-1-R (mouse)

Sequence: SKRGERNFPE EQ

Specificity: The immunizing peptide represents part of an extracellular region.

Cross-Reactivity: Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Pancreas. Recommended concentration: 5 μg/mL.

Western Blot: Approx 55 kDa band observed in Mouse Heart lysates (calculated MW of 53.0 kDa according to NP_067307.2). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GLP1R

Target: GLP1R (Glucagon-Like Peptide 1 Receptor (GLP1R))

Alternative Name: Glp1r

Background: Glp1r, glucagon-like peptide 1 receptor, GLP-1R, GLP1Rc, GLP-1 receptor, GLP-1-R

Gene ID: 14652

NCBI Accession: NP_067307

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, cAMP Metabolic Process, Feeding Behaviour