14-3-3 sigma/SFN antibody (Internal Region) (Biotin)

Catalog No. ABIN5539783

This anti-14-3-3 sigma/SFN antibody is a Goat Polyclonal antibody detecting 14-3-3 sigma/SFN in WB, IHC and ELISA. Suitable for Human and Mouse.

Quick Overview for 14-3-3 sigma/SFN antibody (Internal Region) (Biotin) (ABIN5539783)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This 14-3-3 sigma/SFN antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-14-3-3 sigma/SFN Antibody (Biotin)

Binding Specificity: Internal Region

Purpose: 14-3-3 sigma / Stratifin, Biotinylated

Sequence: CDLHTLSEDS YKDST.

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 28 kDa band observed in Mouse Skin lysates (calculated MW of 27.7 kDa according to Mouse NP_061224.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: SFN

Background: SFN, stratifin, YWHAS, 14-3-3 sigma, epithelial cell marker protein 1

Gene ID: 2810, 55948, 313017

NCBI Accession: NP_006133

Pathways: p53 Signaling, Myometrial Relaxation and Contraction