PYCARD antibody (C-Term) (Biotin)

Catalog No. ABIN5539792

The Biotin-conjugated Goat Polyclonal anti-PYCARD antibody (ABIN5539792) specifically detects PYCARD in WB, ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for PYCARD antibody (C-Term) (Biotin) (ABIN5539792)

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PYCARD antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PYCARD Antibody (Biotin)

Binding Specificity: C-Term

Purpose: ASC / TMS1, Biotinylated

Sequence: CRESQSYLVE DLERS.

Specificity: This antibody is expected to recognise both reported isoforms (NP_037390.2 and NP_660183.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 24 kDa band observed in lysates of cell line U937 (calculated MW of 21.6 kDa according to NP_037390.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PYCARD

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Alternative Name: PYCARD

Background: PYCARD, PYD and CARD domain containing, ASC, CARD5, TMS, TMS-1, TMS1, caspase recruitment domain-containing protein 5, target of methylation-induced silencing 1

Gene ID: 29108

NCBI Accession: NP_037390, NP_660183

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome