YAP1 antibody (Internal Region)

Catalog No. ABIN5539893

Product Details anti-YAP1 Antibody

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Binding Specificity: Internal Region

Reactivity: Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This YAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: YAP1

Sequence: TYHSRDESTD S

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in lysates of cell line MDCK (calculated MW of 50.3 kDa according to Dog XP_536601.2). Data obtained from an anonymous customer. Recommended concentration: 0.5-2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for YAP1

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Alternative Name: YAP1 (YAP1 Products)

Synonyms: YAP antibody, YAP2 antibody, YAP65 antibody, YKI antibody, xyap antibody, yap antibody, yap2 antibody, yap65 antibody, yki antibody, AI325207 antibody, Yap antibody, Yap65 antibody, Yki antibody, Yorkie antibody, cb194 antibody, sb:cb194 antibody, si:ch211-181p1.5 antibody, si:dkey-3b8.3 antibody, wu:fc18c04 antibody, zgc:158380 antibody, Yes associated protein 1 antibody, Yes associated protein 1 S homeolog antibody, yes-associated protein 1 antibody, Yes-associated protein 1 antibody, YAP1 antibody, yap1.S antibody, Yap1 antibody, yap1 antibody

Background: Yes-associated protein 1, 65 kDa, YAP, YAP2, YAP65, Yes-associated protein 1, 65 kD, Yes-associated protein 1, 65 kDa, yes-associated protein 2

Gene ID: 10413, 22601, 363014

NCBI Accession: NP_006097

Pathways: MAPK Signaling, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha