JUP antibody (C-Term) (Biotin)

Catalog No. ABIN5539926

The Goat Polyclonal anti-JUP antibody has been validated for WB and ELISA. It is suitable to detect JUP in samples from Human, Mouse and Rat.

Quick Overview for JUP antibody (C-Term) (Biotin) (ABIN5539926)

Target: JUP (Junction Plakoglobin (JUP))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This JUP antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-JUP Antibody (Biotin)

Binding Specificity: C-Term

Purpose: Plakoglobin / Gamma-catenin, Biotinylated

Sequence: GLRPPYPTAD HMLA.

Specificity: This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 80 kDa band observed in lysates of cell line A431 (calculated MW of 81.7 kDa according to Human NP_002221.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: JUP

Background: JUP, junction plakoglobin, ARVD12, CTNNG, DP3, DPIII, PDGB, PKGB, catenin (cadherin-associated protein), gamma 80 kDa, desmoplakin III, desmoplakin-3

Gene ID: 3728, 16480

NCBI Accession: NP_002221, NP_068831

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location