ACTC1 antibody (N-Term)

Catalog No. ABIN5539979

This Mouse Monoclonal antibody specifically detects ACTC1 in WB, IHC (fro) and EIA. It exhibits reactivity toward Human, Rat, Zebrafish (Danio rerio), Rabbit and Pig.

Quick Overview for ACTC1 antibody (N-Term) (ABIN5539979)

Target: ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))

Reactivity: Human, Rat, Zebrafish (Danio rerio), Rabbit, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACTC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 22D3

Product Details anti-ACTC1 Antibody

Binding Specificity: N-Term

Sequence: Ac-DDEETTALVC -COOH

Specificity: This 22D3 Monoclonal antibody is highly specific for Alpha-Cardiac Actin, and does not cross react with other Actin isoforms.

Purification: Purified

Immunogen: Peptide comprising the N-terminal nonapeptide of alpha-Cardiac Actin with an acetylated N-terminus coupled to keyhole limpet hemocyanin through the cysteine residue.

Isotype: IgG1

Application Details

Application Notes: ELISA. Flow Cytometry. Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Sections. Recommended Dilutions : 1/100-1/1000 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/1000-1/5000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Buffer System: PBS Preservatives: 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.

Target Details for ACTC1

Target: ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))

Alternative Name: alpha 1 cardiac muscle actin,actc1

Background: Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebrates. Cardiac α-actin, which is the main actin isoform in the adult heart, has also been shown to be the predominant form in early muscle development. In later development the expression of α-cardiac actin is down regulated and α-skeletal actin becomes the dominant isoform in the adult skeletal muscle.

Pathways: Myometrial Relaxation and Contraction